In this article we measure the impact of gender on women's legislative behavior by utilizing a unique research design. We compare whether women and men of the same political party represent the same congressional district differently with respect to women's issues. Using bill sponsorship and floor remarks during the 104th to 107th sessions of the U.S. House of Representatives as measures of legislative behavior, we find that female legislators who replace men in the same district introduce more women's issues bills in Congress. Although our conclusion that women legislators represent women's issues more frequently in the House supports existing research, our results do so in a new and more effective way by controlling for the competing explanations of party identification and district opinion as factors determining a legislator's behavior.